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KMID : 0377519970220020063
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Chung-Ang Journal of Medicine
1997 Volume.22 No. 2 p.63 ~ p.75
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Immunohistochemical study on glutathione S-transferase in early skin carcinogenesis by 7,12-dimethylbenzanthracene in the rat
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Park Keum-Min
Song Kye-Yong
Park Sang-Chul
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Abstract
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Glutathione S-transferases GSTs are major detoxcating enzymes which calalyse the process of detoxification of variety of electrophilic compounds in the cytoplasm of most mammalian cells. The GST isoenzymes can be divided into the two major groups basic(alpha) and acidic(pi) form which are abundant in the liver and placenta, respectively. These enzymes are known to be expressed in the neoplastic conditions, especially in the early hepatocarcinogenesis. We carried out the immunohistochemical study on the effects of early skin carcinogenesis by using polyclonal antibodies against keratin, basic GST(GST-L) and acidic GST(GST-P). GST is the major detoxicating enzyme in the skin. The objectives were to observe the changes of the GST in the epidermis during the carcinogenesis provoked with 7,12-dimethylbenzanthracene(DMBA). Histopathologically, phorbol ester induced moderate inflammatory reaction in the dermis but DMBA did not. DMBA alone did not induce no acanthosis. Multiple application of phorbol ester induced mild acanthosis and most pronounced with addition of DMBA. Immumhistochemically, keratin and GST-P espressions were closedly related with acanthosis of epidermis and most pronounced in the skin trated with DMBA and multiple phorbol ester, but GST-L is not induced. GST-P was markedly induced in the early stage of skin carcinogenesis developed by DMBA with phorbol ester and the amounts were closely related with degree of epidermis proliferation. It is, therefore, suggested GST-P play protective roles in the proliferating cells.
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KEYWORD
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glutathione S-tramsferase, skin carcinogenesis, 7, 12-dimethylbenzanthracene
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